Specifically, the substitutions at the 2nd, 15th, and 27th positions may help the peptide resist being broken down by enzymes and extend its half-life
The mechanism may be related to the decreased inflow of iron, TF and TFR1, the upregulation of GPX4, xCT, and FTH1, and the downregulation of ACSL4, MDA and LPO levels
Amino Acids Regulating Skeletal Muscle Metabolism: Mechanisms of Action, Physical Training Dosage Recommendations and Adverse Effects. Nutrition & Metabolism , vol
The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software
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